I was taking between 400-600mg per day. After a couple of weeks, it seemed to be helping. Twitching was decreased and my forearms/hands were not aching. This lasted a couple of months. However, I can’t be sure if it was coincidence or not. When I ran out and stopped taking it in December, I also got a cold at the same time, and a flare up. So I can’t tell if it was the cold or stopping the ALA. Too many variables. I’m giving it a try again.