Chez,I have no specific diagnosed autoimmune disease but have had four ANA titres since this started. The first (about two weeks apart) were EXTREMELY high and the second two several weeks and then many months later were both totally negative. So if I had only been tested once or even twice I may not have even known I had an a lot of autoimmune antibodies. Also, I had a raji test twice that showed high levels of immune complexes. This is also associated with autoimmune diseases although not one specifically. So basically just because you have not been diagnosed with an auotimmue disease or even if you had a few negative autoimmune tests does not rule out that your twitching is related to an autoimmune issue. This also doesn't mean you need to have all the tests I've had if you are doing ok. It just means that this stuff comes and goes and is hard to detect because you blood does not always look the same. Autoimmune processes flare and recess etc.Krackersones