I too have a high ANA 1:320, speckled pattern, but neither my neurologist, nor a rhumetologist that I was referred to, believe that it has anything to do with the muscle twitching. Besides +ANA means very little unless you have other positive immune complexes along with it. If your doctor suspects that something immune is going on, they will test you for other anti-bodies. I get my antibodies checked by my rhumetologist once a year to make sure that I have no other positive ones.... the ANA always comes back positive, but that is it! They actually say a small majority of the population just has a high ANA for no reason...Don't worry too much about it unless your doctor thinks its a problem. I know its hard... I still feel like its this mysterious thing.