I did not notice any difference in twitching after 3 days of niacinamide use. 1000 mg a day. I do take clonopin every day. It does help with the twitching and weird sensations. I am taking 0.75-1mg per day. My psychiatrist wants me to take it everyday for anxiety. At first I didn't, but I do now. I am concerned about long term usage and I am going to ask my neuro for other possible meds to replace it. I have been on it for 2 months. Only side effect is that it makes me tired.